| Congresso Brasileiro de Microbiologia 2023 | Resumo: 381-1 | ||||
Resumo:Barley is a small grain cereal with significant consumption around the world, taking the fifth position of most consumed cereals. For this reason, its quality demands attention from the food industries. Microbiologically, it is susceptible to contamination by filamentous fungi that can cause diseases, leading to lower production and important economic loss. Fungi of the genus Fusarium are among the most reported barley pathogen worldwide and, over the years, the species Fusarium poae has emerged as a barley pathogen and has been commonly reported in different regions from temperate to dry weather. One important issue associated with this species and other ones within the Fusarium genus is the ability to produce mycotoxins, which are secondary metabolites that are toxic for humans and other vertebrates. F. poae can produce some trichothecenes, such as nivalenol (NIV) and diacetoxyscirpenol (DAS), and the maximum levels for these toxins in foodstuffs are not set by the legislations, therefore they are not monitored; nevertheless, studies regarding toxicity and occurrence have demonstrated the growing importance of these toxins, mainly nivalenol. At the level of omics analysis, there are few studies that have monitored this species to understand the pathogen and host relationship, as well as to understand the biosynthetic pathways for mycotoxin production. Based on this information, the present study aimed to investigate the locations and evolution of clustered genes involved in the synthesis of these F. poae mycotoxins, as well as, from a comparative genomic analysis, to analyze the evolutionary relationships between F. poae and other species within the Fusarium sambucinum clade. For that, two strains had their genome sequenced, annotated, and predicted and were submitted to bioinformatics analyses. The findings showed that isolated strains of barley cultivated in Brazil have functional and well-structured clustered genes for the production of NIV and DAS. The analyzes showed greater similarity in the clusters of one of the strains with the reference genome of F. venenatum while the other had greater similarity with the reference of F. poae as expected and, considering that they are two isolates from different geographic regions with a divergent climate, the work may have demonstrated a possible response to the adaptation of the species in the country. Furthermore, potential effector genes responsible for the synthesis of proteins closely related to the pathogenicity of the fungus were also found. Palavras-chave: Secondary metabolism, Cereals, Fusarium sambucinum species complex, Mycotoxins, Fungi genetics Agência de fomento:CAPES, FAPESP | |||||